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How Do We Perform Paternity Testing?
After your sample reaches our laboratory, DNA testing begins immediately on the same day that the samples are received. The DNA test usually takes approximately 5 working days to complete. The testing process can be divided into 4 major stages.
Step 1 - DNA Isolation, Quantitation, and Purification
During this stage, the laboratory isolates the buccal cells from the head of the swabs. After removing the cells from the swabs, the DNA is extracted from the cells. The DNA is quantitated to determine how much DNA is present and the DNA is further concentrated and purified if required.
Step 2 - PCR Amplification
During this stage, the laboratory amplifies specific regions of your DNA for further examination. The DNA is amplified by a process known as PCR (stands for Polymerase Chain Reaction). PCR is a very powerful technique which can amplify a single strand of DNA over a million fold in very little time. PCR is a very sensitive technique and is frequently used in forensic laboratories to analyze blood stains, hair, and saliva stains.
Step 3 - Sequencing
Sequencing allows the laboratory to examine the DNA once it has been amplified by PCR. There are different methods available to detect the amplified DNA. In smaller laboratories, sequencing is often performed manually and is prone to human error. In larger laboratories, sequencing is fully automated. Automated sequencing is fast and very precise. At Genex, sequencing is performed using the latest and most advanced automated sequencers to ensure the most accurate results possible. During sequencing, the DNA fragments are passed through a matrix and detected by lasers. The detected fragments and their properties are then output to a computer for analysis.
Step 4 - Analysis
The final step of the testing process is the analysis of the genetic information. Each and every gene that was examined is fully documented in your final report. If the alleged father is not the true biological father of the child, the alleged father's DNA will not match the half of the child's genetic material which came from the father. If the test man is indeed the true biological father of the child, the paternal half of the genetic material of the child will match that of the alleged father's perfectly. Depending on the race of the tested individuals, a paternity index is generated to determine probability of paternity. At Genex, we examine 16 genetic locations for each individual and we guarantee absolutely conclusive results for every test. All exclusions are 100% and inclusions exceed 99.9%. You will never receive an inconclusive answer.